Référentiel des analyses


référentiel des analyses
référentiel des analyses
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BIOCHIMIE

Analyses

Techniques

Intérêt clinique

Glycémie

Cinétique UV

Suivi et dépistage de diabète

HGPO

Cinétique UV

Suivi et dépistage de diabète

Hémoglobine A1C

HPLC

Suivi de diabète

Peptide C

ECLIA

Evaluation de la sécrétion d’insuline

Insulinémie

Immunoenzymatique

sécrétion d’insuline par le pancréas

Acide urique

Colorimétrique point final

Diagnostic et suivi de la goutte

Micro albumine

Turbidimétrie

Recherche d’une néphropathie diabétique/
Recherche d’un risque cardiovasculaire

Protéinuries de 24h

Turbidimétrie

-

Urée

Cinétique UV

Evaluation de la fonction rénale

Urée urinaire 24H

Cinétique UV

Evaluation de la fonction rénale

Créatinine

Colorimétrique cinétique

Evaluation de la fonction rénale

Créatinine urinaire

Colorimétrique cinétique

Evaluation de la fonction rénale

Créatinine-clairance

Colorimétrique cinétique

Evaluation de la fonction rénale

Chimie des urines

Bandelette

Détecter et de rechercher certaines pathologies

Ionogramme

Potentiométrie

Evaluation de l’équilibre électrolytique

Ionogramme urinaire

Potentiométrie

Evaluation de l’équilibre électrolytique

TGO (ALAT)

Cinétique UV

Exploration des pathologies hépatiques
ou cardiaques

TGP(ASAT)

Cinétique UV

Exploration des pathologies hépatiques

Bilirubine totale

Colorimétrique

Exploration des pathologies hépatiques

Bilirubine direct

Colorimétrique

Exploration des pathologies hépatiques

Gamma glutamyltranspeptidase  (GGT)

Colorimétrique cinétique

Exploration des pathologies hépatiques

Phosphatase alcaline

Cinétique UV

Exploration hépatique ou osseuse

Cholestérol

Colorimétrique point final

Exploration bilan lipidique/
surveillance de traitement hypolipémiant

Triglycérides

Colorimétrique point final

Exploration d’anomalie lipidique /
bilan chez les diabétiques

Cholestérol HDL

Colorimétrique point final

Exploration bilan lipidique/
surveillance de traitement hypolipémiant

Cholestérol LDL

Colorimétrique point final/calcul

Exploration bilan lipidique

Protéines sanguines

Colorimétrique point final

Détection des troubles de l’hydratation, malnutrition

Albumine

Colorimétrique point final

Détection des troubles de l’hydratation, malnutrition

Albumine – urine

Colorimétrique point final

Evaluation de la fonction rénale

Electrophorèse des protéines

Electrophorèse capillaire

Détection des dysprotéinémie, glomérulopathie, hépatites,
syndrome inflammatoires ou infectieux
Déficit immunitaire
Dénutrition

Amylase

Colorimétrique cinétique

Exploration pancréas et glandes salivaires

Lipase

Colorimétrique cinétique

Exploration pancréas

Phosphatase acide prostatique

Colorimétrique cinétique

Surveillance des cancers de la prostate et détection des métastases osseuses

Aldolase

Cinétique UV

Suivi des patients souffrant de dystrophie musculaire,les dermatomyosites

Lactate déshydrogénase LDH

Cinétique UV

Bilan enzymatique (infarctus, infection pulmonaire, pathologie hématologique)

Créatinine kinase  CPK

Cinétique UV

Diagnostic infarctus du myocarde

Créatinine kinase MB CK- MB

Immuno-inhibition

Diagnostic infarctus du myocarde

Troponine

ECLIA/ELFA

Diagnostic précoce et rétrospectif de l’infarctus du myocarde

D-Dimères

ELFA

Thrombose, CIVD, embolie pulmonaire

Vitesse de sédimentation (VS)

méthode de Westergren

Exploration d’un syndrome inflammatoire et en cas de  polyglobulie ou l'anémie et la présence de paraprotéinémie

CRP ultra-sensible

Turbidimétrie

Evaluation de l’inflammation

Calcium

Colorimétrique cinétique

Bilan phospho-calcique

Calcium urinaire

Colorimétrique cinétique

Bilan phospho-calcique

Phosphore

Colorimétrique cinétique

Bilan phospho-calcique

Phosphore urinaire

Colorimétrique cinétique

Bilan phospho-calcique

Magnésium

Colorimétrique cinétique

Détection d’hypomagnésémie

Fer sérique

Colorimétrique cinétique

Diagnostic et surveillance d’anémie microcytaire
et appréciation de la réserve en fer

Ferritine

ECLIA

Capacité de fixation du fer (CTF)

ECLIA/Saturation/précipitation

Coefficient de saturation du fer  (CS)

ECLIA/Saturation/précipitation

Dosage de l’haptoglobine

Turbidimétrie

Dosage de la transferrine

Turbidimétrie

Réaction inflammatoire et recherche d’hémolyse

Electrophorèse d’hémoglobine

Electrophorèse capillaire

Diagnostic d’anémie hémolytique

Glucose 6 phosphate déshydrogènase G6PD

Cinétique UV

Déficit en G6PD

25 –OH Vitamine D (D2+D3)

ELFA

Bilan d’ostéoporose/
évaluation du statut vitaminique

Vitamine B12

ECLIA

Diagnostic d’anémie macrocytaire
et évaluation du statut vitaminique

Folates :Vitamine B9

ECLIA

HORMONOLOGIE

Analyses

Techniques

Intérêt clinique

 

Hormone Thyréostimuline
TSH 3=èmegénération
ultra-sensible

ECLIA/EFLA

Exploration de la fonction thyroïdienne

FT3

ECLIA/EFLA

FT4

ECLIA/EFLA

Thyroglobuline

ECLIA/EFLA

Marqueurs tumoraux de la thyroïde

Calcitonine
(thyro-calcitonine)

ELISA

Détection du carcinome médullaire thyroïdien

 

β Gonadotrophine
chorionique humaine
β  HCG

ECLIA/EFLA

Confirmation de grossesse
Diagnostic et suivi de tumeurs trophoblastiques

Hormone Folliculo Stimulante
 ( FSH)

ECLIA/EFLA

Diagnostic et traitement de la stérilité féminine

Hormone Lutéostimulante  (LH)

ECLIA/EFLA

Diagnostic et traitement de la stérilité féminine

Prolactine

ECLIA/EFLA

Augmentation dans les adénomes à prolactine, les hypothyroïdes, le syndrome des ovaires polykystiques.

Progestérone

ECLIA/EFLA

 Son dosage permet de mettre en évidence l’ovulation et d’apprécier le caractère fonctionnel du corps jaune.

Testostérone

ECLIA/EFLA

Chez l’homme, le taux de testostérone reflète l’activité endocrine des testicules.

Œstradiol

ECLIA/EFLA

Exploration des aménorrhées et/ou de l’infértilité

Dehydro – épiandrostérone sulfate   S-DHEA

ECLIA/EFLA

Exploration androgénique

Delta 4 androsténedione

ELISA

Diagnostic d'un hirsutisme ou du syndrome des ovaires polykystiques.

17 OH progesterone

ELISA

L’intérêt de son dosage réside dans l’exploration des hyperandrogénies liées à un déficit enzymatique surrénalien en 21-hydroxylase.Rythme circadien

Corticothrophine ACTH

ECLIA

Insuffisances corticosurrénaliennes, hyperplasies congénitales des surrénales ou des hypercorticismes, rythme circadien.

ParathyroidehormonePTH intact

EFLA

Bilan phospho-calcique/
suivi chez l’insuffisant rénal/
perturbation parathyroïdienne

Cortisol

ECLIA/EFLA

Bilan hormonal surrénalien/
Surveillance d’une corticothérapie

Insuline

ECLIA

Evaluation de la sécrétion d’insuline

Growth Hormone GH/STH hormone de croissance

ECLIA

Diagnostic d’un retard de croissance (déficit congénital primitif en GH) ou exploration d’une acromégalie.

Hormone anti mullérienne AMH

ECLIA

Marqueur de réserve ovarienne

HEMATOLOGIE

(Numération formule sanguine) FNS

Hémocytomètrie à compteur électronique

Exploration des trois lignées cellulaires :
GR / GB / Plaquette

Frottis sanguin

Microscopique

Etude morphologie  des trois lignée cellulaire : GR / GB / Plaquette

Myélogramme

Microscopique

Diagnostic et suivi d’hémopathie

Adénogramme

Microscopique

Diagnostic d’hémopathie

Réticulocyte

Microscopique

Reflète  l’activité érythropoïétique

Frottis nasal

Microscopique

Détection d’allergie

Test de fragilité osmotique

Hémolyse

Diagnostic de la sphérocytose héréditaire

Temps de saignement (TS)

Chronomètrique

Evaluation de l’hémostase primaire

Taux de prothrombine (TP)

Chronomètrique

Evaluation de la coagulation et suivi de traitement anticoagulant

Temps de céphaline Kaolin (TCK)

Chronomètrique

Evaluation de la coagulation

Taux de Fibrinogène

Chronomètrique

Evaluation de la coagulation

Recherche d’Anticoagulant circulant lupique: Indice de Rosner

Chronomètrique

SAPL, Néoplasies, Lupus, Allongement du TCA, fausses couches à répétitions.

Groupage sanguin

Agglutination

Détermination du groupe sanguin

Test de coombs direct TCD

Agglutination

Diagnostic d’anémie d’origine  immunologique

Test de coombs indirect TCI

Agglutination

Incompatibilité Foeto-Maternelle, maladie hémolytique du nouveau-né, Recherché systématiquement chez les femmes enceintes Rhésus négatif et conjoint rhésus positif

Test auto
anti-érythrocytaire

Agglutination

Détection d’auto-anticorps anti-érythrocytaire

Recherche d’anticorps immuns du système ABO (antiA –antiB)

Agglutination

Ictère néonatal, maladie hémolytique du nouveau née par une immunisation maternelle

IMMUNOLOGIE

Analyses

Techniques

Intérêt clinique

Dosage des Immunoglobuline IgG

Turbidimétrie

Evaluation de l’état immunitaire

Dosage des Immunoglobuline IgA

Turbidimétrie

Evaluation de l’état immunitaire

Dosage des Immunoglobuline IgM

Turbidimétrie

Evaluation de l’état immunitaire

Dosage du complément C3

Turbidimétrie

Evaluation de l’état immunitaire

Dosage du complément C4

Turbidimétrie

Evaluation de l’état immunitaire

Ag CA 125 marqueur tumeur ovarienne

ECLIA/EFLA

Marqueur ovarien

Ag CA 15-3 marqueur tumeur mammaire

ECLIA/EFLA

Marqueur du sein

Ag CA19-9 marqueur tumeur pancreas, colon

ECLIA/EFLA

Marqueur gastro-intestinal

Ag Carcino Embryonnaire (ACE)

ECLIA/EFLA

Marqueur de néoplasie

Alpha foetoprotéine (AFP)

ECLIA/EFLA

Marqueur de carcinomes hépato-cellulaires, tumeurs testiculaires.
Dépistage d’anomalie fœtale chez la femme enceinte

Ag  prostatique (PSA) total

ECLIA/EFLA

Marqueur spécifique de la prostate

Ag  prostatique (PSA) libre

ECLIA/EFLA

Marqueur spécifique de la prostate

Ac anti-thyropéroxydase  ANTI TPO

ECLIA/EFLA

Evaluation des pathologies auto-immunes de la thyroïde

Ac anti-thyroglobulineANTI TG

ECLIA/EFLA

Evaluation des pathologies auto-immunes de la thyroïde

 

Ac anti-récepteur de TSH

ECLIA

Evaluation des pathologies auto-immunes de la thyroïde

Syndrome des anticorps
anti-phospholipide
(SAPL)
IgMcardiolipine
IgGcardiolipine
IgM B2 GP1
IgG B2 GP1

ELISA

Diagnostic du syndrome des anticorps
anti-phospholipides et avortements à répétition

Ac Anti-nucléaire (FAN)
+
Anticorps anti DNA natif
+
Anticorps anti-ANA :  (SSA, SSB, Sm, RNP, J01, Scl 70, Histones, CENPB, PM/Scl, PCNA, Prot.PRib, AMA.M2)

Immuno-Fluorescence Indirecte (IFI)

ELISA

Immuno-Blot

Bilan de connectivite

Anticorps
anti-gliadine  IgA/IgG

ELISA

Diagnostic de la maladie cœliaque

Anticorps
anti-transglutaminase IgA/IgG

ELISA

Anticorps
anti-endomysium

Immuno-Fluorescence Indirecte (IFI)

Anticorps anti-cytoplasmique des polynucléaires (ANCA)
+
profil ANCA :
(PR3, MPO, GBM)

Immuno-Fluorescence Indirecte (IFI)

ELISA

Immuno-Blot

diagnostic de vascularite

Facteur rhumatoïde Waaler-rose

Agglutination

Diagnostic de la Polyarthrite rhumatoïde

 

Facteur rhumatoïde Latex

Agglutination

Facteur rhumatoïde
quantification (IgG)

Turbidimétrie

Anti-CCP
(anti-peptides citrullinés cyclisés)

ELISA

Profil Hépatique
AMA-M2 / LKM1

Immuno-Blot

Diagnostic des hépatites
auto-immunes

Anticorps anti-cellules pariétales

Immuno-Fluorescence Indirecte (IFI)
ELISA

Diagnostic d’anémie de Biermer

Anticorps anti-facteur intrinsèque

Immuno-Fluorescence Indirecte (IFI)

Dosage des Immunoglobuline IgE totaux

ECLIA/EFLA

Diagnostic et suivi des manifestations allergiques

IgE spécifique panel troph-allergène (alimentaire)

IMMUNOBLOT

IgE spécifique panel pneum-allergène (respiratoire)

IMMUNOBLOT

IgE spécifique panel pédiatrique

IMMUNOBLOT

SPERMIOLOGIE

Spermogramme
+
spermo-cytogramme

Cytologieautomatisé

Appréciation de la fertilité masculine

Spermoculture

Cytologie / culture

Recherche d’infection urétrale

SEROLOGIE

Analyses

Techniques

Intérêt clinique

Anti HCV Hépatite C

ECLIA/EFLA/ELISA

Diagnostic de l’hépatite virale C

Anti HIV (Human Immunodéficience Virus)

ECLIA/EFLA

Diagnostic de l’infection à l’HIV

Ag HBs Hépatite B

ECLIA/EFLA

Diagnostic et suivi  d’hépatite B

Anti-HBs Hépatite B

ECLIA/EFLA

Anti-HBcIgM Hépatite B

ECLIA/EFLA

Ag HBe Hépatite B

ECLIA/EFLA

Anti-HBe Hépatite B

ECLIA/EFLA

HAVA b IgMHépatite A

ECLIA/EFLA

Rubéole IgG

ECLIA/EFLA/ELISA

Statut immunitaire vis-à-vis la rubéole /surveillance chez la femme enceinte

Rubéole IgM

ECLIA/EFLA/ELISA

Toxoplasmose IgM

ECLIA/EFLA/ELISA

Statut immunitaire vis-à-vis la rubéole /surveillance chez la femme enceinte

Toxoplasmose IgG

ECLIA/EFLA/ELISA

Cytomégalovirus (CMV)  IgG

ECLIA/EFLA/ELISA

Diagnostic infection auau Cytomégalovirus-IgG

Cytomégalovirus CMV IgM

ECLIA/EFLA/ELISA

Statut immunitaire vis-à-vis du CMV

MNI
(Mononucléose infectieuse)

Immunochromatographie

Diagnostic rapide de mononucléose infectieuse

 Antistreptolysine O  (ASLO)

Turbidimétrie

Suivi sérologique d’une infection à streptocoque A

HelicobacterpyloriIgG

Bandelette

Dépistage  de la gastrite chronique à H.Pylori

Sérodiagnostic de
Widal et Félix

Agglutination

Diagnostic sérologique de la fièvre typhoïde à salmonella typhi

TPHA /BW/VDRL (tréponematose)

Immunochromatographie

/ECLIA/ELISA
Diagnostic de la syphilis

Brucellose
(dépistage + titrage)

Agglutination

Diagnostic de la brucellose

Anti-chlamydia Trachomatis
IgG ou IgA

ELISA

Infection ancienne ou récente à Chlamydia trachomatis

Anti-chlamydia TrachomatisIgM

ELISA

Infection récente à Chlamydia trachomatis

Mycoplasme Urogenitaux Sérologie

Inhibition de croissance

Infection à mycoplasmes urogénitaux

Hydatitose

Hémagglutination indirecte

Diagnostic de l’hydatidose

MICROBIOLOGIE

Analyses

Techniques

Intérêt clinique

Examen Cytobacteriologique des Urines (ECBU)

Cytologie

Recherche d’infection urinaire

HLM
(compte d’ADDIS)

Cytologie

Infection urinaire, Tuberculose urinaire, néphropathie, glomérulopathie, atteinte des voies excrétrices

Coprologie des selles

Cytologie / culture

infections bactériennes du tube digestif

Recherche de sang dans les selles

Immunochimiques

Dépistage de la présence d’hémoglobine érythrocytaire provenant d’hémorragies
gastro-intestinales

Prélèvement vaginal

Cytologie / culture

Recherche d’infection vaginale

Prélèvement urétral

Cytologie / culture

Recherche d’infection urétrale

Ag chlamydia Trachomatis

Immunochromatographie

Uréthrites ou salpingites à, chlamydia, infertilité

Mycoplasme Urogenitaux antigène

Cytologie / culture

Infection à mycoplasmes urogénitaux

Examen cytobactériologique  Ascite

Cytologie / culture

Infection du liquide d’Ascite

Examen cytobactériologique liquide articulaire

Cytologie / culture

Infection du liquide articulaire

Examen cytobactériologique liquide pleural

Cytologie / culture

Infection du  liquide pleural

Examen bactériologique du prélèvement nasal

Cytologie / culture

Infection nasal

Examen bactériologique du prélèvement auriculaire

Cytologie / culture

Infection auriculaire

PARASITILOGIE-MYCOLOGIE

Parasitologie des selles

Cytologie / culture

Parasitoses intestinales

Scotch test

Cytologie

Dépistage de l’oxyurose

Examen parasitologique vaginale

Cytologie

Trichomonose

Examen mycologique
cytologie, culture

Cytologie / culture

Infection mycologique

PHARAMACO-TOXICOLOGIE

Acide Valproique (Dépakine)

ECLIA

Surveillance de traitement
antiépileptique

Carbamazépine (Tégrétol)

ECLIA

Surveillance de traitement antiépileptique

 

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