PLATEFORME TECHNIQUE

Le plateau technique du laboratoire assure une activité de biologie polyvalente comprenant les analyses de : biochimie, hématologie, hormonologie, immunologie, sérologie (bactérienne, virale et parasitaire), la microbiologie et parasitologie.

Tous nos automates sont reliés en réseau avec le logiciel du laboratoire Biolab®Analysis.

PREANALYTIQUE

La pré-analytique comprend toutes les étapes allant de la prescription à la réalisation de l'analyse et implique différents acteurs.

Parmi les différents facteurs qui peuvent influencer un résultat d'analyse, certains tels que l'âge, le poids, le sexe, les facteurs génétiques, le cycle menstruel, le type d'alimentation, l'exercice physique ne sont pas maîtrisables au moment du prélèvement mais doivent être connus.

D'autres facteurs sont maîtrisables :

  • la préparation du patient et sa posture.
  • l'heure du prélèvement
  • la pose d'un garrot
  • le choix du tube adéquat et l'ordre de prélèvement de ces derniers
  • la prise d'un médicament.
  • l'identification précise du patient, de son ordonnance et de ses tubes (nom, prénom, date de naissance, examen demandé)
  • la vitesse de centrifugation, et la décantation du sérum
  • le délai de réalisation de l’examen.

Une formation a été effectuée au personnel d’accueil et au personnel de prélèvements sur les facteurs influençant la pré-analytique, afin de garantir des résultats fiables, et d’améliorer le service rendu aux patients.

BIOCHIMIE

Les analyses de BIOCHIMIE sont réalisées sur Les automates suivants :

  • Automates INTEGRA 400 Roche
  • Automates VITROS 350 Ortho clinical diagnostics
  • Automate d10 Biorad
  • 03 Automates prolyte
  • Capillarys Flex Percing Sebia
  • Automate adams arkray

Ces automates utilisant les techniques suivantes:

La potentiométrie, spectrophotométrie, néphélémétrie, la turbidimétrie, HPLC, enzymatique.

Ces techniques qui permettent le dosage dans le sang et dans l'urine des bilans biochimies suivants:

- Exploration biochimique du rein :urée, créatinine, clairances, acide urique, protéinuries, micro-albuminurie

- Exploration biochimique du foie :ASAT, ALAT, PAL, GGT, bilirubine.

- Métabolisme Phosphocalcique et magnésium : calcium, phosphore.

- Exploration biochimique du cœur et des anomalies lipidiques : CPK, CK-MB,  troponines, triglycérides, cholestérol, HDL, LDL.

- Exploration biochimique du fer : fer, ferritine, transferrine, coefficient de saturation(CS), capacité de fixation du fer (CTF).

- Exploration biochimique du diabète: glucose, HGPO, hémoglobine glyquée, insulinémie, peptide C.

- Métabolisme hydro-électrolytique : sodium, potassium, chlore

- Profil protéique: taux de protéines, albumine, CRP.

- Enzymologie :LDH, Lipase, amylase, phosphatase acide, G6PD.

- Marqueurs tumoraux: PSA total, PSA libre, AFP, ACE, CA 125, CA 15-3, CA 19-9.

- Facteurs anti-pernicieux : vitamine B12, acide folique.

HORMONOLOGIE

Les analyses d’hormonologie sont réalisées sur les automates suivants :

  • 02 Automates E411 Roche
  • Automate Access II Beckman
  • Automate Vidas biomérieux

Ces automates utilisant les techniques suivantes : Electro-chimie-Lumeniscence (ECLIA), Enzyme linked fluorescence assay (ELFA), Enzyme Linked ImmunoSorbent Assay (ELISA).

Les hormones à doser se rangent en deux catégories : petites molécules, type : les molécules stéroïdiennes, ou grandes molécules, type : les hormones protéiques.

- Bilanthyroidien :TSH, FT3, FT4, thyroglobuline, calcitonine.

- Axe somatotrope : GH

- Axe corticotrope : cortisol, ACTH

- Fértilité et grossesse : B HCG, FSH, LH, prolactine, testosterone, oestradiol, progesterone,  DHES, 17 OH progesterone, AMH, delta4 androstenedione

- Regulation phospho-calcique :PTH, VIT D

- Régulation glycémique :  insuline, peptide C

HEMATOLOGIE

Les analyses d’hématologie sont réalisées sur les automates suivantes :

  • Coulter Médonic
  • Coulter Quintus
  • Coagulomètre randox
  • Coagulométre Bio solea 2
  • Capillarys Flex Percing Sebia
  • Microscope optique

Ces automates utilisant les techniques suivantes : Hémocytomètrie à compteur électronique, Chronomètrique, Agglutination, microscopique.

Cytologie : FNS, Frottis sanguin, Taux de réticulocytes, Myélogramme, Adénogramme, frottis nasal, test de fragilité osmotique.

Biochimie de l’hémoglobine : électrophorèse de l’hémoglobine

Hemostose : TP TCK Fibrinogéne

Immuno-hematologie : groupage sanguin, TCD, TCI, test auto, recherche d’hémolysine du systéme ABO.

IMMUNOLOGIE

Les analyses d’immunologie sont réalisées sur les automates suivantes :

  • Automate d' ELISA DS 2
  • Automate CHORUS DIESSE
  • Microscope d’immunofluoresence
  • Lecteur d’immunodot

Ces automates utilisant les  techniques suivantes: Immuno-Fluorescence Indirecte, Immuno-Blot, ELISA, Immunochromatographie.

Bilan d’auto-immunité : ANTI TPO, ANTI TG, FAN ,Anti DNA , facteurs rhumatoides, Anti CCP , ANCA , Profil Hépatique , Sérologie de la maladie cœliaque , recherche Facteur intrinsèque , recherche Anti Cellule Pariétale,  Anticorps Anti-Phospholipides (S.A.P.L).
Dosage spécifiques des Protéines sériques et électrophorèse de Protéines: CRP,C3,C4,Haptoglobine,Transferrine,IgG , IgAet IgM, électrophorèse de Protéines.
Allergologie : dosage des IgE Totale et spécifiques ( pannel Alimentaire , respiratoire et Pédiatrique ).

 

SEROLOGIE

Les analyses de  sérologie sont réalisées sur les automates suivantes :

  • Automate Ds2
  • Automate E411
  • Automate ACCESS II Bekcman
  • Automate Vidas

 Ces automates utilisant les techniques suivantes : Electro-chimie-Lumeniscence (ECLIA),enzyme linked fluorescence assay (ELFA), Enzyme Linked ImmunoSorbent Assay (ELISA), Hémaglutination indirecte, Agglutination, immunochromatographie.

Sérologie bactérienne : sérologie de Widal et felix, sérologie de tréponème, Brucellose, ASLO, Helicobacter pylori, Chlamydia Trachomatis, Mycoplasme urogénitaux

Sérologie virale : HCV, HBV, HIV, HAV, MNI, CMV, Rubéole

Sérologie parasitaire : Toxoplasmose, Hydatidose.

MICROBIOLOGIE

Nous prenons en charge les différents prélèvements :

- ECB des Urines,  automatisé par l'analyseur iQ®200 ELITE  Iris®
- ECB du Sperme: spermoculture .
- ECB du prélèvement Vaginal.

- Recherche de Gonocoque, Mycoplasme urogénitaux et Chlamydia trachomatis dans : prélèvement urétral et vaginal.
- Coproculture .

PARASITOLOGIE ET MYCOLOGIE

- parasitologie des selles.
- recherche de Trichomonas Vaginalis dans le sperme et le prélèvement vaginal.
- Recherche de Candida dans les différents prélèvements (urines, pus, gorge, sperme, prélèvement vaginal)

SPERMIOLOGIE 

Nous prenons en charge:
Spermogramme et Spemocytogramme : complétement automatisé.
Spermoculture, Recherche Chalmydia Trachomatis et Mycoplasme urogénitaux.

Certaines analyses très spécialisées ne sont pas réalisées par LAM ALFARABI. Elles sont sous-traitées par un Laboratoires spécialisés reconnus : BIOMNIS, et avec lequel une convention est signée selon la réglementation en vigueur.

Lorsqu’une analyse est sous-traitée, le patient en est informé systématiquement

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